Plikaytis B B, Marden J L, Crawford J T, Woodley C L, Butler W R, Shinnick T M
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
J Clin Microbiol. 1994 Jun;32(6):1542-6. doi: 10.1128/jcm.32.6.1542-1546.1994.
In 1991, a multidrug-resistant strain of Mycobacterium tuberculosis was isolated from eight people with tuberculosis at a state correctional facility in New York. This strain, which is designated strain W (IS6110 restriction fragment length polymorphism type 212072), was resistant to isoniazid, rifampin, ethambutol, streptomycin, kanamycin, ethionamide, and rifabutin. Since that outbreak, the W strain has been associated with outbreaks in five hospitals in the New York City area and is a continuing public health problem in the area. To be able to identify this strain rapidly, we developed a multiplex PCR assay which targets a direct repeat of IS6110 with a 556-bp intervening sequence (NTF-1). The amplification generates two amplicons from strain W, which indicate the presence and orientation of the NTF-1 sequence between the direct repeat of IS6110, and a third amplicon, which serves as an internal PCR control. The assay was evaluated with 193 isolates of M. tuberculosis, and all 48 strain W isolates among those 193 isolates were correctly identified.
1991年，在纽约的一所州立惩教机构中，从8名结核病患者身上分离出一株耐多药结核分枝杆菌。该菌株被命名为W菌株（IS6110限制性片段长度多态性类型212072），对异烟肼、利福平、乙胺丁醇、链霉素、卡那霉素、乙硫异烟胺和利福布汀耐药。自那次疫情爆发以来，W菌株已与纽约市地区的五家医院的疫情相关联，并且仍是该地区持续存在的公共卫生问题。为了能够快速鉴定该菌株，我们开发了一种多重PCR检测方法，该方法靶向IS6110的一个直接重复序列，其间隔序列为556 bp（NTF-1）。扩增从W菌株产生两个扩增子，这表明IS6110直接重复序列之间NTF-1序列的存在和方向，以及第三个扩增子，其用作内部PCR对照。用193株结核分枝杆菌分离株对该检测方法进行了评估，在这193株分离株中的所有48株W菌株分离株均被正确鉴定。
