Puolakkainen M, Parker J, Kuo C C, Grayston J T, Campbell L A
Department of Pathobiology, University of Washington, Seattle 98195, USA.
Microbiol Immunol. 1995;39(8):551-4. doi: 10.1111/j.1348-0421.1995.tb02241.x.
Studies using monoclonal antibodies have demonstrated species-specific reactivities with Chlamydia pneumoniae. In this study, further characterization of C. pneumoniae specific monoclonal antibodies TT-205 and RR-402 and description of C. pneumoniae specific antibodies prepared against other isolates are presented. TT-205 and RR-402 were shown to neutralize infectivity. Neutralization in cell culture was specific and enhanced by complement. Attempts to characterize the reactive antigen by immunoblotting, immunoaffinity chromatography and radioimmunoprecipitation were unsuccessful, probably due to difficulties in solubilizing the immunoreactive epitope without denaturing it. Recognition of the determinant by the monoclonal antibodies is labile to physical and chemical treatments suggesting that the reactive epitope is conformational.
使用单克隆抗体的研究已证明与肺炎衣原体存在种属特异性反应。在本研究中，对肺炎衣原体特异性单克隆抗体TT - 205和RR - 402进行了进一步表征，并描述了针对其他分离株制备的肺炎衣原体特异性抗体。已证明TT - 205和RR - 402可中和感染性。细胞培养中的中和作用具有特异性且可被补体增强。通过免疫印迹、免疫亲和层析和放射免疫沉淀对反应性抗原进行表征的尝试均未成功，这可能是由于在不使其变性的情况下难以溶解免疫反应性表位。单克隆抗体对该决定簇的识别对物理和化学处理不稳定，这表明反应性表位是构象性的。
