Wen H, Craig P S, Ito A, Vuitton D A, Bresson-Hadni S, Allan J C, Rogan M T, Paollilo E, Shambesh M
Department of Biological Sciences, University of Salford, U.K.
Ann Trop Med Parasitol. 1995 Oct;89(5):485-95. doi: 10.1080/00034983.1995.11812981.
Antigen binding of total-IgG and IgG-subclass antibodies from patients with alveolar or cystic echinococcosis (AE and CE) was assessed by immunoblotting. Antigen extracts were prepared from Echinococcus multilocularis protoscoleces (EmP) or from homogenized E. multilocularis metacestode tissue (EmCH). Antigens of approximately 44, 35, 21, 17.5 and 16.5 were recognized by total-IgG and IgG1- and IgG4-subclass antibodies in some of 50 human AE sera from China, Japan or France. The 44- and 35-kDa polypeptides, present in both EmP and EmCH extracts, were recognized by total-IgG antibodies in sera from 82% and 66% of the AE patients, respectively. However, over 30% cross-reactivity occurred between these two antigens and sera from CE and Taenia solium cysticercosis patients. The immunoblot specificities of the 27-, 21- and 17.5-kDa antigens in EmP for E. multilocularis infection were 73%, 88% and 93%, respectively. Recognition of the 17.5-kDa antigen in the EmP immunoblot was much higher for the Japanese AE cases (11/13; 85%) than for the French (9/19; 47%) or Chinese (9/18; 50%) AE cases. None of the CE cases from Uruguay or Libya, where human AE has not been reported, was seropositive for the 17.5-kDa antigen. Antibodies from three (7.3%) of the 41 Chinese CE cases recognized the 17.5-kDa antigen. Within the 13 Japanese AE sera, the combined detection by IgG1, IgG4 and total-IgG antibodies of the 27-, 21- and 17.5-kDa antigens in either EmP or EmCH immunoblots was greater than that by each class/subclass alone, increasing the overall sensitivity for AE patients. A combined ELISA/immunoblot approach, including IgG-subclass detection using E. multilocularis protocolex or cyst extracts, could be useful for the differential diagnosis of human alveolar echinococcosis. An algorithm for such an approach is given.
通过免疫印迹法评估肺泡型或囊型棘球蚴病（AE和CE）患者的总IgG及IgG亚类抗体的抗原结合情况。抗原提取物由多房棘球绦虫原头节（EmP）或多房棘球绦虫幼虫匀浆组织（EmCH）制备。在中国、日本或法国的50份人类AE血清中，部分血清的总IgG、IgG1和IgG4亚类抗体识别出了约44、35、21、17.5和16.5 kDa的抗原。EmP和EmCH提取物中均存在的44 kDa和35 kDa多肽，分别在82%和66%的AE患者血清中被总IgG抗体识别。然而，这两种抗原与CE和猪带绦虫囊尾蚴病患者血清之间出现了超过30%的交叉反应。EmP中27 kDa、21 kDa和17.5 kDa抗原对多房棘球绦虫感染的免疫印迹特异性分别为73%、88%和93%。日本AE病例（11/13；85%）在EmP免疫印迹中对17.5 kDa抗原的识别率远高于法国（9/19；47%）或中国（9/18；50%）的AE病例。在乌拉圭或利比亚（未报告人类AE病例）的CE病例中，无一例对17.5 kDa抗原呈血清学阳性。41例中国CE病例中有3例（7.3%）的抗体识别出了17.5 kDa抗原。在13份日本AE血清中，IgG1、IgG4和总IgG抗体联合检测EmP或EmCH免疫印迹中的27 kDa、21 kDa和17.5 kDa抗原的检出率高于各亚类单独检测，提高了对AE患者的总体敏感性。一种包括使用多房棘球绦虫原头节或囊肿提取物检测IgG亚类的联合ELISA/免疫印迹方法，可能有助于人类肺泡型棘球蚴病的鉴别诊断。给出了这种方法的算法。
