Ito A, Ma L, Itoh M, Cho S Y, Kong Y, Kang S Y, Horii T, Pang X L, Okamoto M, Yamashita T, Lightowlers M W, Wang X G, Liu Y H
Department of Parasitology, Gifu University School of Medicine, Japan.
Clin Diagn Lab Immunol. 1997 Jan;4(1):57-9. doi: 10.1128/cdli.4.1.57-59.1997.
An improved enzyme-linked immunosorbent assay (ELISA) system using partially purified Eml8/16 enriched fraction (PP-Em18/16) prepared by isoelectric focusing was evaluated for serodiagnosis of alveolar echinococcosis (AE). The PP-Em18/16-ELISA was compared with Em2plus-ELISA by using sera from AE and cystic echinococcosis (CE) patients in China, where both AE and CE are endemic; sera from CE patients in Australia, where only CE exists; and sera from patients with cysticercosis, paragonimiasis, or sparganosis in Korea, where no indigenous AE or CE exists. We used Em2plus-ELISA as a standard ELISA and found 24.6% (17 of 69 specimens) cross-reactivity with sera from CE. Furthermore, some of the sera from paragonimiasis, sparganosis, and cysticercosis patients were also cross-reactive in the Em2plus-ELISA. When we tested for similar cross-reactivity in the same sera from CE patients by PP-Em18/16-ELISA (23.2%, 16 of 69), it became evident that the specificity of the PP-Em18/16-ELISA was better than that of the Em2plus-ELISA, since no sera from patients with the examined parasitic diseases except CE showed cross-reactivity. Some CE patients from China showed exceptionally high levels of antibody in comparison with those of CE patients from Australia, where no AE occurs. It is speculated that these patients with strongly positive cases of CE from China may have been exposed to both species of Echinococcus.
评估了一种改进的酶联免疫吸附测定（ELISA）系统，该系统使用通过等电聚焦制备的部分纯化的富含Eml8/16的级分（PP-Em18/16）用于肺泡型棘球蚴病（AE）的血清学诊断。在中国，AE和囊性棘球蚴病（CE）均为地方病，使用AE和CE患者的血清将PP-Em18/16-ELISA与Em2plus-ELISA进行比较；在仅存在CE的澳大利亚，使用CE患者的血清；在韩国，不存在本土的AE或CE，使用囊尾蚴病、肺吸虫病或裂头蚴病患者的血清。我们将Em2plus-ELISA作为标准ELISA，发现与CE患者的血清有24.6%（69份标本中的17份）交叉反应。此外，一些来自肺吸虫病、裂头蚴病和囊尾蚴病患者的血清在Em2plus-ELISA中也有交叉反应。当我们通过PP-Em18/16-ELISA对CE患者的相同血清进行类似的交叉反应测试时（23.2%，69份中的16份），很明显PP-Em18/16-ELISA的特异性优于Em2plus-ELISA，因为除CE外，所检查的寄生虫病患者的血清均未显示交叉反应。与澳大利亚（无AE发生）的CE患者相比，一些来自中国的CE患者显示出异常高的抗体水平。据推测，这些来自中国的CE强阳性病例患者可能同时接触了两种棘球绦虫。
