用Bal 31核酸酶处理透化细胞以切除染色体DNA环。
Excision of chromosomal DNA loops by treatment of permeabilised cells with Bal 31 nuclease.
作者信息
Lagarkova M A, Iarovaia O V, Razin S V
机构信息
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
出版信息
Mol Gen Genet. 1995 Nov 15;249(2):253-6. doi: 10.1007/BF00290373.
PMID:7500948
Abstract
The specificity of long-range fragmentation of human nucleolar genes by Bal 31 nuclease was studied using fractionation of cleavage products by pulsed field gel electrophoresis, followed by Southern hybridization. It was found that limited treatment of permeabilised cells with this nuclease results in accumulation of DNA scissions in matrix attachment areas. Consequently, chromosomal DNA loops and their oligomers are released from the genome.
摘要
利用脉冲场凝胶电泳对切割产物进行分级分离，随后进行Southern杂交，研究了Bal 31核酸酶对人类核仁基因的长程片段化特异性。结果发现，用这种核酸酶对通透细胞进行有限处理会导致基质附着区域积累DNA断裂。因此，染色体DNA环及其寡聚物从基因组中释放出来。
Zotero 插件