Pellequer J L, Westhof E
UPR Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance, Institut de Biologie Moléculaire et Cellulaire du CNRS, France.
J Mol Graph. 1993 Sep;11(3):204-10, 191-2. doi: 10.1016/0263-7855(93)80074-2.
A program (PREDITOP) for predicting the location of antigenic regions (or epitopes) on proteins is described. This program and the associated ones are written in Turbo Pascal and run on IBM-PC compatibles. The program contains 22 normalized scales, corresponding to hydrophilicity, accessibility, flexibility, or secondary structure propensities. New scales are easily implemented. An hydrophobic moment procedure has also been implemented in order to determine amphiphilic helices. The program generates a result file where the values represent a particular physicochemical aspect of the studied protein. PREDITOP can display one or several result files by simple graphical super-imposition. Curve combinations can be done by the ADDITIO or MULTIPLI routines which create a new result file by adding or multiplying previously calculated files representing several propensities. The program is useful and efficient for identifying potential antigenic regions in a protein with the aim of raising antibodies against synthesized peptides which cross-react with the native protein.
本文描述了一个用于预测蛋白质上抗原区域（或表位）位置的程序（PREDITOP）。该程序及相关程序用Turbo Pascal编写，可在IBM-PC兼容机上运行。该程序包含22个归一化量表，分别对应亲水性、可及性、柔韧性或二级结构倾向。新的量表很容易实现。还实施了一种疏水矩程序以确定两亲性螺旋。该程序生成一个结果文件，其中的值代表所研究蛋白质的特定物理化学方面。PREDITOP可以通过简单的图形叠加显示一个或多个结果文件。曲线组合可以通过ADDITIO或MULTIPLI例程完成，这些例程通过将代表几种倾向的先前计算文件相加或相乘来创建一个新的结果文件。该程序对于识别蛋白质中的潜在抗原区域很有用且高效，目的是产生针对与天然蛋白质交叉反应的合成肽的抗体。
