Mowatt M R, Nguyen B Y, Conrad J T, Adam R D, Nash T E
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Infect Immun. 1994 Apr;62(4):1213-8. doi: 10.1128/iai.62.4.1213-1218.1994.
Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.
蓝氏贾第鞭毛虫通过调节构成滋养体变体特异性表面蛋白（VSP）库的不同基因的表达来进行抗原变异。我们研究了一个表位，该表位在源自独立的蓝氏贾第鞭毛虫分离株WB、G3M、Be-2和CAT的克隆滋养体系所表达的VSP中是保守的。单克隆抗体6E7识别的表位完全位于这些大小可变的VSP所特有的串联重复65个氨基酸单位的区域内。Northern（RNA）杂交、cDNA克隆和DNA序列分析表明，这些VSP之间的大小异质性是由于重复单位数量的差异所致。
