Kubota T, Sato K, Kabuto M, Kawano H, Nakagawa T, Arai Y, Tachibana O, Yoshida K, Yamashita J
Department of Neurosurgery, Fukui Medical School.
Noshuyo Byori. 1994;11(1):23-8.
Eight chordomas were studied by using specific antibodies against cytokeratin, vimentin, desmin, type II collagen, S-100 protein, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP). One chondrosarcoma was included as a representative of chondroid tumors. All chordomas were positively stained for epithelial markers (cytokeratin and EMA), whereas the chondrosarcoma was not. All chordomas and the chondrosarcoma expressed vimentin, type II collagen and S-100 protein. Oncofetal antigens (CEA and AFP) and desmin were found in five (63%) chordomas, whereas these antigens were not found in the chondrosarcoma. Ultrastructurally the chordoma cells showed junctional complexes, microvillous projections and basal lamina-like structures alongside the tumor cells, whereas the tumor cells of the chondrosarcoma had neither junctions and microvillous projections, nor basement membrane. The present study demonstrates the utility of these tumor markers and ultrastructural features in the differential diagnosis of chordomas and tumors with similar histologic patterns such as chondrosarcomas.
使用针对细胞角蛋白、波形蛋白、结蛋白、II型胶原蛋白、S-100蛋白、上皮膜抗原（EMA）、癌胚抗原（CEA）和甲胎蛋白（AFP）的特异性抗体对8例脊索瘤进行了研究。纳入1例软骨肉瘤作为软骨样肿瘤的代表。所有脊索瘤上皮标志物（细胞角蛋白和EMA）呈阳性染色，而软骨肉瘤则否。所有脊索瘤和软骨肉瘤均表达波形蛋白、II型胶原蛋白和S-100蛋白。在5例（63%）脊索瘤中发现了癌胚抗原（CEA）和甲胎蛋白（AFP）以及结蛋白，而在软骨肉瘤中未发现这些抗原。超微结构上，脊索瘤细胞在肿瘤细胞旁可见连接复合体、微绒毛突起和基底膜样结构，而软骨肉瘤的肿瘤细胞既无连接和微绒毛突起，也无基底膜。本研究证明了这些肿瘤标志物和超微结构特征在脊索瘤与组织学模式相似的肿瘤（如软骨肉瘤）鉴别诊断中的作用。
