Brodin E, Rosén A, Schött E, Brodin K
Department of Pharmacology, Karolinska Institute, Stockholm, Sweden.
Neuropeptides. 1994 Apr;26(4):253-60. doi: 10.1016/0143-4179(94)90079-5.
The effect of specific stressful stimuli on neuropeptide levels was studied in rat brain regions known to be involved in the mediation of stress responses and anxiety. Rats were sequentially removed, one by one with 20-min intervals from group cages and immediately decapitated. A selective increase of the somatostatin level was observed in the amygdala in the rats taken for sacrifice second last and last, compared to the rats taken earlier from the respective group cage (increases by 40 to 69%, p < 0.05 or p < 0.01). Isolation of rats in single cages for 24 h or 1 week before sacrifice, increased the substance P level in the dorsal periaqueductal grey by 26 and 27% (p < 0.05 in both cases), respectively, compared to group housed rats. In group housed rats treated with diazepam (5 mg/kg, s.c.) 140 min before sacrifice, the level of substance P in the rostral hippocampus and dorsal periaqueductal grey was reduced by 40% (p < 0.001) and 28% (p < 0.05), respectively, compared to saline treated controls. In conclusion, handling, as well as a single dose of the anxiolytic drug diazepam, appears to induce rapid, selective and region-specific changes of regional brain peptide levels in the rat. The effects of handling are likely to be related to the acute stress response and are probably not secondary to increased plasma glucocorticoid levels.
在已知参与应激反应和焦虑调节的大鼠脑区中，研究了特定应激刺激对神经肽水平的影响。将大鼠从群居笼中依次取出，每隔20分钟取出一只，然后立即断头处死。与较早从各自群居笼中取出的大鼠相比，在倒数第二只和最后一只用于处死的大鼠杏仁核中观察到生长抑素水平有选择性增加（分别增加40%至69%，p<0.05或p<0.01）。在处死前将大鼠单独饲养在单笼中24小时或1周，与群居大鼠相比，中脑导水管周围灰质背侧的P物质水平分别增加了26%和27%（两种情况均p<0.05）。在处死前140分钟用安定（5mg/kg，皮下注射）处理的群居大鼠中，与生理盐水处理的对照组相比，海马喙部和中脑导水管周围灰质背侧的P物质水平分别降低了40%（p<0.001）和28%（p<0.05）。总之，处理以及单剂量的抗焦虑药物安定似乎会在大鼠中诱导脑区肽水平快速、选择性和区域特异性的变化。处理的影响可能与急性应激反应有关，可能不是血浆糖皮质激素水平升高的继发结果。
