Roosendaal R, Kuipers E J, van den Brule A J, Peña A S, Uyterlinde A M, Walboomers J M, Meuwissen S G, de Graaff J
Department of Clinical Microbiology, Free University Hospital, Amsterdam, The Netherlands.
J Clin Microbiol. 1994 Apr;32(4):1123-6. doi: 10.1128/jcm.32.4.1123-1126.1994.
A 16S ribosomal DNA-based PCR appeared to be a sensitive test for the detection of infection by Helicobacter pylori in 31 patients when compared with culturing and histological and serological techniques. For five patients, PCR was the only test with a positive result. H. pylori DNA was also found in gastrointestinal equipment even after standard intensive combined manual and machine cleaning. We therefore conclude that a reliable validation of PCR for the detection of H. pylori in gastric biopsy specimens is possible only when the cleaning and disinfection method used has been proven to remove all H. pylori DNA from gastrointestinal equipment. An adequate cleaning and disinfection method for the removal of H. pylori DNA from fiberoptic endoscopes is described.
与培养、组织学和血清学技术相比，基于16S核糖体DNA的聚合酶链反应（PCR）似乎是检测31例患者幽门螺杆菌感染的一种敏感方法。对于5例患者，PCR是唯一呈阳性结果的检测方法。即使经过标准的强化人工和机器联合清洁，在胃肠道设备中也发现了幽门螺杆菌DNA。因此，我们得出结论，只有当所使用的清洁和消毒方法已被证明能从胃肠道设备中去除所有幽门螺杆菌DNA时，才有可能对用于检测胃活检标本中幽门螺杆菌的PCR进行可靠验证。本文描述了一种从纤维内镜中去除幽门螺杆菌DNA的充分清洁和消毒方法。
