Viale A M, Arakaki A K, Soncini F C, Ferreyra R G
Departmento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Int J Syst Bacteriol. 1994 Jul;44(3):527-33. doi: 10.1099/00207713-44-3-527.
The essential GroEL proteins represent a subset of molecular chaperones ubiquitously distributed among species of the eubacterial lineage, as well as in eukaryote organelles. We employed these highly conserved proteins to infer eubacterial phylogenies. GroEL from the species analyzed clustered in distinct groups in evolutionary trees drawn by either the distance or the parsimony method, which were in general agreement with those found by 16S rRNA comparisons (i.e., proteobacteria, chlamydiae, bacteroids, spirochetes, firmicutes [gram-positive bacteria], and cyanobacteria-chloroplasts). Moreover, the analysis indicated specific relationships between some of the aforementioned groups which appeared not to be clearly defined or controversial in rRNA-based phylogenetic studies. For instance, a monophyletic origin for the low-G+C and high-G+C subgroups among the firmicutes, as well as their specific relationship to the cyanobacteria-chloroplasts, was inferred. The general observations suggest that GroEL proteins provide valuable evolutionary tools for defining evolutionary relationships among the eubacterial lineage of life.
必需的GroEL蛋白是分子伴侣的一个子集，广泛分布于真细菌谱系的物种以及真核细胞器中。我们利用这些高度保守的蛋白来推断真细菌的系统发育。所分析物种的GroEL在通过距离法或简约法绘制的进化树中聚为不同的组，这与通过16S rRNA比较所发现的结果总体一致（即变形菌门、衣原体、拟杆菌、螺旋体、厚壁菌门[革兰氏阳性菌]和蓝细菌-叶绿体）。此外，分析表明上述一些类群之间存在特定关系，这些关系在基于rRNA的系统发育研究中似乎没有得到明确界定或存在争议。例如，推断出厚壁菌门中低G+C和高G+C亚群的单系起源，以及它们与蓝细菌-叶绿体的特定关系。总体观察结果表明，GroEL蛋白为确定生命的真细菌谱系之间的进化关系提供了有价值的进化工具。
