Gu Q Z, He J G, Chen H
Hangzhou Branch, Zhejiang Medical University.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(4):270-2.
Immunoenzymatic staining technique (IEST) using PVC-membrane coated with Schistosoma japonicum ultrasonicated dry egg (UDE) and liver egg PVC-IEST was carried out to detect specific antibodies in sera from 159 cases with schistosomiasis japonica, the positive rates being 98.1% (156/159) and 97.5% (155/159), respectively. The sera from 63 healthy individuals showed false positive reaction in 2 cases and 1 case, respectively, suggesting that the sensitivity and specificity of the two tests were similar. However, the positive intensity was higher in UDE section than in liver egg section (P > 0.05). As the UDE section could be stained more obvious and the PVC membranes are cheap and could be easily brought so that this method might be applicable for practical purposes.
采用涂有日本血吸虫超声干卵（UDE）的聚氯乙烯（PVC）膜和肝卵PVC免疫酶染色技术（IEST）检测159例日本血吸虫病患者血清中的特异性抗体，阳性率分别为98.1%（156/159）和97.5%（155/159）。63名健康个体的血清分别有2例和1例出现假阳性反应，表明两种检测方法的敏感性和特异性相似。然而，UDE切片的阳性强度高于肝卵切片（P>0.05）。由于UDE切片染色更明显，且PVC膜价格便宜且易于携带，因此该方法可能适用于实际应用。
