Gu Q Z, He J G, Chen H
Hangzhou Branch, Zhejiang Medical University.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1992;10(1):59-61.
Using PVC membrane instead of common slide in liver egg-immunoenzymatic staining technique (IEST) for diagnosis of schistosomiasis japonica to detect specific antibodies in sera from 179 cases with schistosomiasis japonica, the positive rate was 97.2% (174/179). Sera from 63 healthy individuals revealed in one positive (1.5%). No cross reaction was observed in sera from 6 cases of paragonimiasis. PVC-IEST and SLIDE-IEST were comparatively performed on sera from 78 cases with schistosomiasis japonica, the positive rates were 96.2% (75/78) and 97.4% (76/78), respectively (P greater than 0.05). As PVC-IEST is simpler to perform than SLIDE-IEST, it is suggested that the former is more suitable for application in the field for diagnostic purposes.
在日本血吸虫病诊断的肝虫卵免疫酶染色技术（IEST）中，使用聚氯乙烯（PVC）膜代替普通载玻片检测179例日本血吸虫病患者血清中的特异性抗体，阳性率为97.2%（174/179）。63名健康个体的血清中有1例呈阳性（1.5%）。6例肺吸虫病患者的血清未观察到交叉反应。对78例日本血吸虫病患者的血清进行PVC-IEST和载玻片-IEST对比检测，阳性率分别为96.2%（75/78）和97.4%（76/78）（P＞0.05）。由于PVC-IEST比载玻片-IEST操作更简便，建议前者更适合在现场用于诊断目的。
