Game J, Bell M, Ramotar D, Miller H
Life Sciences Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
Yeast. 1994 Apr;10(4):543-54. doi: 10.1002/yea.320100414.
We have used the previously described technique of random-breakage mapping to locate the two yeast genes APN1 and YUH1. The APN1 locus is located approximately 235 kb from the left telomere of chromosome XI, and shows weak (approximately 53 cM) genetic linkage to ura1. The YUH1 locus is located approximately 140 kb from the right telomere of chromosome X, and genetically maps 3.6 cM distal to cdc11. In addition, we show by random-breakage mapping that TRP3 is located approximately 45 kb from the left telomere of chromosome XI, whereas FAS1 is approximately 110 kb from the same telomere. This supports a gene order on the left distal portion of chromosome XI that agrees with other physical reports but is inverted with respect to Edition 11 of the published genetic map. This report confirms that random-breakage mapping is a rapid and convenient method of locating cloned genes.
我们运用了先前描述的随机断裂图谱技术来定位酵母的两个基因APN1和YUH1。APN1基因座位于离第十一条染色体左末端约235 kb处，并且与ura1表现出较弱的（约53 cM）遗传连锁。YUH1基因座位于离第十条染色体右末端约140 kb处，在遗传图谱上位于cdc11远端3.6 cM处。此外，我们通过随机断裂图谱表明，TRP3位于离第十一条染色体左末端约45 kb处，而FAS1离同一末端约110 kb。这支持了第十一条染色体左远端部分的基因顺序，该顺序与其他物理报告一致，但与已发表的遗传图谱第11版相反。本报告证实，随机断裂图谱是定位克隆基因的一种快速且便捷的方法。
