Briere R O
CRC Crit Rev Clin Lab Sci. 1978;10(1):1-30.
At the time of admission, 4 to 8% of all hospital admissions have a elevation of alkaline phosphatase. This high incidence of alkaline phosphatasemia has resulted in not only a need to identify the source of the elevated alkaline phosphatase; it has also led to a marked proliferation in laboratory procedures aimed at identifying the various isoenzymes of alkaline phosphatase. These various techniques are reviewed and their relative merits are discussed. No single technique adequately delineates all of the alkaline phosphatase isoenzymes, but the combination of heat fractionation, electrophoresis, and the measurement of an additional hepatobiliary enzyme has been shown to be able to identify the source of origin of more than 95% of all cases of alkaline phosphatasemia. The individual isoenzymes of alkaline phosphatase are discussed in detail, and the underlying pathophysiology is reviewed so that physiologic elevations of alkaline phosphatase are not confused with pathologic increases in alkaline phosphatase.
入院时，所有住院患者中有4%至8%的人碱性磷酸酶升高。碱性磷酸酶血症的高发生率不仅导致需要确定碱性磷酸酶升高的来源；还导致旨在鉴定碱性磷酸酶各种同工酶的实验室检测方法显著增多。本文对这些不同的技术进行了综述，并讨论了它们的相对优点。没有一种单一技术能够充分区分所有碱性磷酸酶同工酶，但热分级分离、电泳以及测量另一种肝胆酶相结合已被证明能够识别超过95%的碱性磷酸酶血症病例的来源。本文详细讨论了碱性磷酸酶的各个同工酶，并对潜在的病理生理学进行了综述，以便碱性磷酸酶的生理性升高不会与病理性升高相混淆。
