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用于鉴定石蜡包埋组织中幽门螺杆菌的原位杂交

In situ hybridisation for the identification of Helicobacter pylori in paraffin wax embedded tissue.

作者信息

Bashir M S, Lewis F A, Quirke P, Lee A, Dixon M F

机构信息

Department of Histopathology, General Infirmary, Leeds.

出版信息

J Clin Pathol. 1994 Sep;47(9):862-4. doi: 10.1136/jcp.47.9.862.

DOI:10.1136/jcp.47.9.862
PMID:7525660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC494950/
Abstract

A method for identifying Helicobacter pylori using a non-isotopic in situ hybridisation technique is described. A probe generated by polymerase chain reaction (PCR) with primers directed against parts of the Helicobacter pylori 16SrRNA sequence was used. Paraffin wax embedded gastric biopsy specimens from patients with and without gastritis were hybridised with the probe, and the method was shown to be sensitive and specific for H pylori.

摘要

描述了一种使用非同位素原位杂交技术鉴定幽门螺杆菌的方法。使用通过聚合酶链反应（PCR）生成的探针，该探针的引物针对幽门螺杆菌16SrRNA序列的部分区域。将来自有胃炎和无胃炎患者的石蜡包埋胃活检标本与该探针杂交，结果表明该方法对幽门螺杆菌具有敏感性和特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dce7/494950/de0882637ccd/jclinpath00222-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dce7/494950/de0882637ccd/jclinpath00222-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dce7/494950/de0882637ccd/jclinpath00222-0091-a.jpg

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本文引用的文献

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Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa.评估从胃黏膜内镜活检标本中分离幽门弯曲菌的培养技术。
J Clin Pathol. 1985 Oct;38(10):1127-31. doi: 10.1136/jcp.38.10.1127.
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Detection of Campylobacter pylori DNA by hybridisation with non-radioactive probes in comparison with a 32P-labelled probe.与32P标记探针相比，用非放射性探针杂交检测幽门螺杆菌DNA。
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Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients.通过活检尿素酶试验检测幽门螺杆菌：对1445例患者的评估
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Oligonucleotide probe for detection and identification of Campylobacter pylori.用于检测和鉴定幽门螺杆菌的寡核苷酸探针
J Clin Microbiol. 1989 Dec;27(12):2652-5. doi: 10.1128/jcm.27.12.2652-2655.1989.
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Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.用于检测人和动物幽门螺杆菌的直接聚合酶链反应试验
J Clin Microbiol. 1991 Nov;29(11):2543-9. doi: 10.1128/jcm.29.11.2543-2549.1991.