Brummer-Korvenkontio H, Palosuo T, François G, Reunala T
National Public Health Institute, Helsinki, Finland.
Int Arch Allergy Immunol. 1997 Feb;112(2):169-74. doi: 10.1159/000237450.
Mosquito bites cause immediate wheals and delayed bite papules in sensitized subjects having saliva-specific IgE and IgG4 antibodies. At present, mosquito saliva antigens are not well characterized.
To identify immunogenic proteins in mosquito saliva and study their cross-reactivity we immunized mice with Aedes communis, Aedes aegypti and Anopheles stephensi bites. Immune sera were used in immunoblotting and immunoblot inhibition experiments.
The main A. communis saliva antigens were 22-, 30-, and 36-kD, A. aegypti saliva antigens 31-, 36-, 46- and 64- to 66-kD, and A. stephensi saliva antigen 46-kD proteins. Most of the saliva antigens appeared to be species-specific and only weak cross-reactivity was observed with heterologous immune sera. Distinct cross-reactivity was observed only between saliva proteins of A. communis and Aedes punctor, two taxonomically closely related species. Human IgE and IgG4 antibodies from mosquito-bite-sensitive children bound to the same saliva proteins as antibodies from the immunized animals.
This study disclosed several immunogenic proteins in Aedes and Anopheles mosquito saliva and suggests that these proteins can also be allergenic in man.
蚊子叮咬会在具有唾液特异性IgE和IgG4抗体的敏感个体中引起即刻风团和延迟性叮咬丘疹。目前，蚊子唾液抗原的特征尚不明确。
为了鉴定蚊子唾液中的免疫原性蛋白并研究它们的交叉反应性，我们用普通伊蚊、埃及伊蚊和斯氏按蚊的叮咬免疫小鼠。免疫血清用于免疫印迹和免疫印迹抑制实验。
普通伊蚊唾液的主要抗原为22kD、30kD和36kD，埃及伊蚊唾液抗原为31kD、36kD、46kD以及64至66kD，斯氏按蚊唾液抗原为46kD蛋白。大多数唾液抗原似乎具有种特异性，用异种免疫血清仅观察到微弱的交叉反应性。仅在普通伊蚊和刺扰伊蚊（两个分类学上密切相关的物种）的唾液蛋白之间观察到明显的交叉反应性。来自对蚊子叮咬敏感儿童的人IgE和IgG4抗体与免疫动物的抗体结合相同的唾液蛋白。
本研究揭示了伊蚊和按蚊唾液中的几种免疫原性蛋白，并表明这些蛋白在人类中也可能具有致敏性。
