Gushchin I S, Prozorovsky N S, Ignatyeva E M, Chitaeva V G, Leskov V P
Institute of Immunology, Ministry of Public Health, Moscow, Russia.
Agents Actions. 1994 Jun;41 Spec No:C16-8. doi: 10.1007/BF02007747.
It has been shown that peripheral blood mononuclear cells (PBMC) obtained from atopic patients with acute clinical manifestations of pollinosis, atopic dermatitis or bronchial asthma, and preincubated in vitro for 18 hours, acquired the ability to induce histamine release from auto-basophils and basophils of healthy donors. Both PBMC and their supernatants possessed this histamine releasing activity (HRA). During remission, HRA could be reproduced in sensitive patients after positive cutaneous tests with a specific allergen. Skin tests with non-specific allergen or histamine-induced provocations were ineffective. HRA of PBMC was also reproduced in healthy individuals after pronounced Prausnitz-Küstner reactions or compound 48/80-induced inflammatory responses. It is concluded that the in vivo activation of mast cells (MC) might be responsible for the acquirement by PBMC of the potential ability to induce histamine release and that this ability was realized after in vitro incubation of such prepared PBMC.
研究表明，从患有花粉症、特应性皮炎或支气管哮喘急性临床表现的特应性患者中获取的外周血单个核细胞（PBMC），在体外预孵育18小时后，获得了诱导健康供体自身嗜碱性粒细胞和嗜碱性粒细胞释放组胺的能力。PBMC及其上清液均具有这种组胺释放活性（HRA）。在缓解期，对敏感患者进行特异性变应原皮肤阳性试验后，可重现HRA。用非特异性变应原进行皮肤试验或组胺诱导激发无效。在明显的普劳斯尼茨-屈斯特纳反应或48/80化合物诱导的炎症反应后，健康个体中也可重现PBMC的HRA。结论是，肥大细胞（MC）的体内激活可能是PBMC获得诱导组胺释放潜在能力的原因，并且这种能力在体外孵育此类制备的PBMC后得以实现。
