Thueson D O, Speck L S, Lett-Brown M A, Grant J A
J Immunol. 1979 Aug;123(2):626-32.
Supernatants from 1- to 2-day cultures of human mononuclear cells induced the release of histamine from basophils. Generation of this histamine-releasing activity (HRA) was stimulated by addition of concanavalin A to the cell cultures. Mononuclear cells were also cultured with SKSD and Candida albicans antigens. Stimulation of HRA production by these antigens was correlated with positive delayed skin reactions. Serial dilutions of supernatants assayed for HRA provided a semiquantitative determination of the level of HRA in mitogen- or antigen-stimulated samples. Antigen increased HRA production when added during the first or second day of culture. Generation of HRA probably requires active protein synthesis, since puromycin was inhibitory, and since preformed HRA could not be recovered from lysed cells. HRA was detected in supernatants after 4 hr, and the effects of antigen stimulation were apparent after 8 hr of culture. Replacement of supernatants with fresh culture medium allowed continued synthesis of substantial quantities of HRA during the second day of culture. A linear correlation was observed between the amount of HRA produced and the mononuclear cell concentration. Our findings provide evidence for the interaction of lymphocytes and basophils via a soluble mediator.
人单核细胞1至2天培养物的上清液可诱导嗜碱性粒细胞释放组胺。向细胞培养物中添加伴刀豆球蛋白A可刺激这种组胺释放活性（HRA）的产生。单核细胞也用SKSD和白色念珠菌抗原进行培养。这些抗原对HRA产生的刺激与阳性迟发性皮肤反应相关。对HRA进行检测的上清液系列稀释可对有丝分裂原或抗原刺激样本中的HRA水平进行半定量测定。在培养的第一天或第二天添加抗原时，抗原会增加HRA的产生。HRA的产生可能需要活跃的蛋白质合成，因为嘌呤霉素具有抑制作用，而且从裂解细胞中无法回收预先形成的HRA。培养4小时后在上清液中检测到HRA，培养8小时后抗原刺激的效果明显。在培养的第二天，用新鲜培养基替换上清液可使大量HRA持续合成。观察到产生的HRA量与单核细胞浓度之间存在线性相关性。我们的研究结果为淋巴细胞和嗜碱性粒细胞通过可溶性介质相互作用提供了证据。
