Fink L M, Hsu P L
Pathology and Laboratory Medicine Service, John L. McClellan Memorial Veterans Hospital, Little Rock, AR 72205.
Virchows Arch. 1994;424(1):1-6. doi: 10.1007/BF00197385.
New concepts and methodologies that can be used to generate proteins, such as specific variable regions of immunoglobulins and other binding peptides in an in vitro selection system are reviewed. These technologies can also be used to alter the kinetics, affinity and avidity of various binding interactions. The nature of epitopes recognized by specific antibodies or receptors can be delineated using selected epitopes displayed on bacteriophages. The basic principles of the technology is predicted upon the belief that if one has a large enough variety of keys, one can open any given lock. The range of utility of these systems to generate new reagents will impact upon the development of new diagnostic and therapeutic reagents. This technology should allow for a much wider range of probes which may have increased binding capacity and allow the development of more sensitive assays with higher signal to noise ratios. These reagents can be produced more efficiently without the use of animals and will be used in diagnostic and experimental pathology. This brief review presents a concise description of the concepts and uses of this new technology. Selected references and reviews are given as sources for further details.
本文综述了可用于生成蛋白质的新概念和方法，例如体外选择系统中免疫球蛋白的特定可变区和其他结合肽。这些技术还可用于改变各种结合相互作用的动力学、亲和力和avidity（亲和力，此处原文可能有误，应为avidity）。使用展示在噬菌体上的选定表位可以描绘特定抗体或受体识别的表位的性质。该技术的基本原理基于这样一种信念，即如果一个人拥有足够多样的“钥匙”，就可以打开任何给定的“锁”。这些系统生成新试剂的效用范围将影响新诊断和治疗试剂的开发。这项技术应该能够提供范围更广的探针，这些探针可能具有更高的结合能力，并允许开发具有更高信噪比的更灵敏的检测方法。这些试剂可以在不使用动物的情况下更高效地生产，并将用于诊断和实验病理学。这篇简短的综述简要描述了这项新技术的概念和用途。给出了选定的参考文献和综述作为获取更多详细信息的来源。
