Bari F, Jancsó G
Department of Physiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Eur Arch Otorhinolaryngol. 1994;251(5):287-92. doi: 10.1007/BF00181887.
Mechanisms of capsaicin-induced vascular changes were examined in the nasal mucosa of anesthetized adult rats. Intra-arterial infusions of capsaicin at doses of 20-100 pmol/min into the external carotid artery resulted in a dose-dependent increase in nasal blood flow as assessed by laser-Doppler flowmetry. Intra-arterial infusion of ruthenium red (RR, 2.5-10 mumol) prior to the administration of capsaicin significantly inhibited the capsaicin-evoked response. The technique of vascular labelling was used to examine nasal mucosal vascular permeability. Intravenous administration of colloidal silver solution prior to capsaicin infusion resulted in accumulation of colloid in the walls of small blood vessels, indicative of enhanced vascular permeability. Vascular labelling was largely abolished after RR pretreatment. These findings suggest that neuropeptides released from trigeminal sensory nerve endings play a significant role in the local vascular and inflammatory reactions of the nasal mucosa. The experimental approach utilized in this study provides a promising model for defining the roles of capsaicin-sensitive afferent nerves in the mechanisms of allergic and/or inflammatory diseases affecting the nasal mucosa.
在麻醉成年大鼠的鼻黏膜中研究了辣椒素诱导血管变化的机制。通过激光多普勒血流仪评估，以20 - 100 pmol/分钟的剂量对外颈动脉进行动脉内输注辣椒素，会导致鼻血流量呈剂量依赖性增加。在给予辣椒素之前动脉内输注钌红（RR，2.5 - 10 μmol）可显著抑制辣椒素诱发的反应。采用血管标记技术检测鼻黏膜血管通透性。在输注辣椒素之前静脉注射胶体银溶液，导致胶体在小血管壁中积聚，表明血管通透性增强。RR预处理后，血管标记在很大程度上被消除。这些发现表明，三叉神经感觉神经末梢释放的神经肽在鼻黏膜的局部血管和炎症反应中起重要作用。本研究中使用的实验方法为确定辣椒素敏感传入神经在影响鼻黏膜的过敏性和/或炎症性疾病机制中的作用提供了一个有前景的模型。
