Böhm H R, Tischendorf F W
Klin Wochenschr. 1978 Jan 15;56(2):61-7. doi: 10.1007/BF01480085.
Cationic lysosomal proteins such as cationic leukocyte antigen (CLA) and lysozyme were estimated in leukocyte lysates of acute and chronic leukaemics using quantitative enzymatic and immunoprecipitation techniques. The studies demonstrate that both lysosomal conponents are markers of non-lymphatic leukaemias. Therefore the determination of CLA and lysozyme is valuable for the differential diagnosis of acute leukaemias. The intracellular cationic protein contents showed characteristic kinetic variations as showing during X-irradiation of the spleen in cases of chronic myelocytic leukaemia. White cells in leukocytosis could be distinguished from normals by remarkably low cationic protein contents in leukocyte lysates. The results are interpreted in the light of current results revealed by immunofluorescent analyses.
使用定量酶法和免疫沉淀技术，对急性和慢性白血病患者白细胞裂解物中的阳离子溶酶体蛋白，如阳离子白细胞抗原（CLA）和溶菌酶进行了测定。研究表明，这两种溶酶体成分都是非淋巴细胞白血病的标志物。因此，CLA和溶菌酶的测定对急性白血病的鉴别诊断具有重要价值。在慢性粒细胞白血病患者脾脏接受X线照射期间，细胞内阳离子蛋白含量呈现出特征性的动态变化。白细胞增多症患者白细胞裂解物中的阳离子蛋白含量显著低于正常水平，据此可将其与正常人区分开来。根据免疫荧光分析的现有结果对这些结果进行了解释。
