Matsuhashi H, Onodera T
Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
Jikken Dobutsu. 1994 Oct;43(4):471-5. doi: 10.1538/expanim1978.43.4_471.
Mouse renal juxtaglomerular cells (JGC) were vitally stained with neutral red, and then fixed with glutaraldehyde to study by electron microscopy. Ultrastructural observation showed a large number of characteristic low electron dense juxtaglomerular granules (JGG) or low dense granules (LDG) in the cytoplasm of JGC. Many LDG contained a flocculent material with a dense core, which was as dense as the contents of JGG. Two kinds of LDG were observed. One of LDG was surrounded by single membrane and partial membrane, and another was surrounded by double membranes. Some LDG did not contain electron dense flocculent material. LDG were surrounded by rough endoplasmic reticulum (r-ER). Alteration of Golgi apparatus and r-ER was observed in JGC. Golgi complex did not associate with JGG and LDG.
用中性红对小鼠肾近球细胞（JGC）进行活体染色，然后用戊二醛固定，以进行电子显微镜研究。超微结构观察显示，JGC细胞质中有大量特征性的低电子密度近球颗粒（JGG）或低密度颗粒（LDG）。许多LDG含有带致密核心的絮状物质，其密度与JGG的内容物一样高。观察到两种LDG。一种LDG被单膜和部分膜包围，另一种被双膜包围。一些LDG不含电子致密絮状物质。LDG被粗面内质网（r-ER）包围。在JGC中观察到高尔基体和r-ER的改变。高尔基体复合体与JGG和LDG不相关。
