Washimi O, Ueda R, Ariyoshi Y, Suyama M, Seki T, Takahashi T, Takahashi T
Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Nagoya.
Jpn J Cancer Res. 1994 Nov;85(11):1112-6. doi: 10.1111/j.1349-7006.1994.tb02915.x.
CD44 is a cell surface receptor that has been implicated in lymphocyte homing, hematopoiesis, cell migration and possibly also tumor metastasis. In the present study, expression of CD44 variant (CD44v) isoforms was analyzed in 23 lung cancer specimens together with corresponding normal lung tissues by Southern blot analysis coupled with reverse transcription-polymerase chain reaction amplification. We found that CD44v isoforms were expressed in all lung cancer specimens, suggesting a possible role in the establishment of metastases by these highly malignant tumors, but normal tissues were also positive. This is in marked contrast to the previous reports of essentially negligible expression of CD44v isoforms in normal colon and breast, and suggests a physiological function in the lung.
CD44是一种细胞表面受体，与淋巴细胞归巢、造血作用、细胞迁移以及可能的肿瘤转移都有关联。在本研究中，通过Southern印迹分析结合逆转录-聚合酶链反应扩增，对23例肺癌标本及其相应的正常肺组织中CD44变异体（CD44v）亚型的表达进行了分析。我们发现，所有肺癌标本中均表达CD44v亚型，这表明这些高恶性肿瘤在转移形成过程中可能发挥作用，但正常组织也呈阳性。这与之前关于正常结肠和乳腺中CD44v亚型表达基本可忽略不计的报道形成了显著对比，提示其在肺中具有生理功能。
