Saalmüller A, Aasted B, Canals A, Dominguez J, Goldman T, Lunney J K, Maurer S, Pescovitz M D, Pospisil R, Salmon H
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
Vet Immunol Immunopathol. 1994 Oct;43(1-3):219-28. doi: 10.1016/0165-2427(94)90139-2.
Fifty-four mAb preselected in the first round of the first porcine CD workshop for their possible reactivity with T-lymphocyte specific antigens and/or activation antigens were further analysed in a second round. PBMC, thymocytes and nylon-wool purified T lymphocytes derived from peripheral blood, mesenteric lymph nodes and spleen served as target cells for flow cytometric analyses. For the classification of activation antigens several experiments were performed with activated, mitogen-stimulated T lymphocytes and long-term T-lymphocyte cultures. Out of the 54 mAb, 35 mAb could be distributed to six different CD clusters and two swine workshop clusters (SWC). Five mAb could be distributed to the porcine CD2, four mAb to the CD4. Six mAb seemed to recognize the porcine CD5 and two mAb the porcine CD6 analogue. Six mAb were directed against the porcine CD8, whereas two different epitopes could be defined. One mAb was directed against the porcine CD25 analogue. Nine mAb could be clustered to the SWC1, defining an antigen on T lymphocytes and cells of the myeloic linage. Two mAb with high T-cell specificity were clustered to the SWC2.
在第一轮猪CD研讨会上预先筛选出的54种单克隆抗体（mAb），因其可能与T淋巴细胞特异性抗原和/或活化抗原发生反应，在第二轮中进行了进一步分析。来自外周血、肠系膜淋巴结和脾脏的外周血单核细胞（PBMC）、胸腺细胞和经尼龙毛纯化的T淋巴细胞用作流式细胞术分析的靶细胞。为了对活化抗原进行分类，对活化的、有丝分裂原刺激的T淋巴细胞和长期T淋巴细胞培养物进行了多项实验。在这54种单克隆抗体中，35种单克隆抗体可分为六个不同的CD簇和两个猪研讨会簇（SWC）。5种单克隆抗体可归为猪CD2，4种单克隆抗体归为CD4。6种单克隆抗体似乎识别猪CD5，2种单克隆抗体识别猪CD6类似物。6种单克隆抗体针对猪CD8，可定义两种不同的表位。1种单克隆抗体针对猪CD25类似物。9种单克隆抗体可归为SWC1，定义了T淋巴细胞和髓系谱系细胞上的一种抗原。两种具有高T细胞特异性的单克隆抗体归为SWC2。
