Stanley J, Linton D, Burnens A P, Dewhirst F E, On S L, Porter A, Owen R J, Costas M
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Microbiology (Reading). 1994 Dec;140 ( Pt 12):3441-9. doi: 10.1099/13500872-140-12-3441.
Campylobacter-like organisms were isolated from the liver, duodenum and caecum of broiler and layer chickens, and from humans with gastroenteritis. They formed a unique DNA homology group and a polyphasic taxonomic analysis was made of 16 strains. Analysis of the nucleotide sequence of the 16S rRNA gene from seven of the strains identified them as belonging to a single species, within the genus Helicobacter. This conclusion was supported by the studies of relative DNA homology and of total protein electrophoretic patterns. The new species could be biochemically differentiated from other helicobacters and its ultrastructure in the electron microscope was typical of the genus except that the flagellum was not sheathed. We propose the name Helicobacter pullorum sp. nov. for this group. Like H. fennelliae or H. cinaedi it represents another non-gastric urease-negative Helicobacter species colonizing the lower bowel. Its isolation from the livers of chickens with vibrionic hepatitis is significant. We describe a species-specific PCR assay for H. pullorum sp. nov. which will facilitate its identification and further studies of its epidemiology.
从肉鸡和蛋鸡的肝脏、十二指肠和盲肠以及患有肠胃炎的人类体内分离出了类弯曲杆菌。它们形成了一个独特的DNA同源群，并对16株菌株进行了多相分类分析。对其中7株菌株的16S rRNA基因核苷酸序列分析表明，它们属于螺杆菌属中的一个单一物种。这一结论得到了相对DNA同源性研究和总蛋白电泳图谱研究的支持。该新物种在生化特性上可与其他螺杆菌区分开来，其在电子显微镜下的超微结构是该属的典型特征，只是鞭毛没有鞘膜。我们提议将这个菌群命名为幽门螺杆菌新种（Helicobacter pullorum sp. nov.）。与芬内利螺杆菌（H. fennelliae）或西奈螺杆菌（H. cinaedi）一样，它代表了另一种在肠道下段定殖的非胃脲酶阴性螺杆菌物种。从患有弧菌性肝炎的鸡肝脏中分离出该菌具有重要意义。我们描述了一种针对幽门螺杆菌新种的种特异性PCR检测方法，这将有助于对其进行鉴定以及进一步开展其流行病学研究。
