Gulati P, Chan A S, Leong S K
Department of Anatomy, National University of Singapore, Kent Ridge.
Cell Tissue Res. 1995 Feb;279(2):405-9. doi: 10.1007/BF00318498.
Nicotinamide-adenine-dinucleotide phosphate-diaphorase positive cells in the chick thymus were studied at the electron-microscopic level. The formazan, a marker for the enzyme nitric oxide synthase, labelled cystic, undifferentiated, endocrine-like and myoid cells in the medulla. Some lymphoid and reticulo-epithelial cells were also lightly labelled. The reaction product was predominantly bound to the membranes of the endoplasmic reticulum in all the cells labelled and also to the nuclear envelope and outer membrane of mitochondria. The Golgi apparatus and the plasma membrane were free of the reaction product.
在电子显微镜水平下研究了雏鸡胸腺中烟酰胺腺嘌呤二核苷酸磷酸黄递酶阳性细胞。作为一氧化氮合酶标记物的甲臜，标记了髓质中的囊性、未分化、内分泌样和肌样细胞。一些淋巴细胞和网状上皮细胞也有轻度标记。在所有标记的细胞中，反应产物主要结合在内质网的膜上，也结合在核膜和线粒体的外膜上。高尔基体和质膜没有反应产物。
