Zhao H, Firestein S, Greer C A
Section of Neurobiology and Neurosurgery, Yale University School of Medicine, New Haven, CT 06510, USA.
Neuroreport. 1994 Dec 30;6(1):149-52. doi: 10.1097/00001756-199412300-00038.
NADPH-diaphorase staining was studied in the intact olfactory system and following transections of the olfactory nerve. Intense staining was found in the olfactory epithelia of rat and salamander, especially at the mucosal surface. The olfactory bulb nerve and glomerular layers were also stained. Transection of the olfactory nerve in salamander, or bulbectomy in rat, resulted in decreased staining at the mucosal surface, although staining increased in cells deeper in the epithelium. Deafferentation also caused the disappearance of staining in the olfactory nerve and glomerular layers of the bulb. These results support the notion of a role for NADPH in the olfactory system, perhaps as a biosynthesis substrate for NO modulation of cGMP in the developing epithelium and a complementary role for CO in the adult.
在完整的嗅觉系统以及嗅神经横断后，对NADPH - 黄递酶染色进行了研究。在大鼠和蝾螈的嗅上皮中发现了强烈的染色，尤其是在黏膜表面。嗅球神经层和肾小球层也被染色。蝾螈嗅神经横断或大鼠嗅球切除后，黏膜表面的染色减少，尽管上皮深层细胞的染色增加。去传入神经也导致嗅球嗅神经和肾小球层的染色消失。这些结果支持了NADPH在嗅觉系统中起作用的观点，可能作为发育中上皮细胞中NO调节cGMP的生物合成底物，以及在成体中CO的互补作用。
