Fox J G, Yan L L, Dewhirst F E, Paster B J, Shames B, Murphy J C, Hayward A, Belcher J C, Mendes E N
Division of Comparative Medicine, Massachusetts Institute of Technology Cambridge 02139.
J Clin Microbiol. 1995 Feb;33(2):445-54. doi: 10.1128/jcm.33.2.445-454.1995.
A fusiform bacterium with 3 to 14 multiple bipolar sheathed flagella and periplasmic fibers wrapped around the cell was isolated from the liver, bile, and lower intestine of aged, inbred mice. The bacteria grew at 37 and 42 degrees C under microaerophilic conditions, rapidly hydrolyzed urea, were catalase and oxidase positive, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and were resistant to both cephalothin and nalidixic acid but sensitive to metronidazole. On the basis of 16S rRNA gene sequence analysis, the organism was classified as a novel helicobacter, Helicobacter bilis. This new helicobacter, like Helicobacter hepaticus, colonizes the bile, liver, and intestine of mice. Although the organism is associated with multifocal chronic hepatitis, further studies are required to ascertain whether H. bilis is responsible for causing chronic hepatitis and/or hepatocellular tumors in mice.
从老年近交系小鼠的肝脏、胆汁和下肠道中分离出一种梭形细菌，该细菌具有3至14根多根双极鞘鞭毛，且有周质纤维包裹细胞。该细菌在微需氧条件下于37℃和42℃生长，能快速水解尿素，过氧化氢酶和氧化酶呈阳性，可将硝酸盐还原为亚硝酸盐，不水解吲哚乙酸或马尿酸盐，对头孢噻吩和萘啶酸均耐药，但对甲硝唑敏感。基于16S rRNA基因序列分析，该微生物被归类为一种新型幽门螺杆菌——胆汁幽门螺杆菌。这种新型幽门螺杆菌与肝螺杆菌一样，定殖于小鼠的胆汁、肝脏和肠道。尽管该微生物与多灶性慢性肝炎有关，但仍需进一步研究以确定胆汁幽门螺杆菌是否导致小鼠慢性肝炎和/或肝细胞肿瘤。
