Fox J G, Drolet R, Higgins R, Messier S, Yan L, Coleman B E, Paster B J, Dewhirst F E
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139, USA.
J Clin Microbiol. 1996 Oct;34(10):2479-82. doi: 10.1128/jcm.34.10.2479-2482.1996.
On the basis of biochemical, phenotypic, and 16S rRNA analysis, a novel gram-negative bacterium, isolated from normal and diarrheic dogs as well as humans with gastroenteritis, has been recently named Helicobacter canis. A 2-month-old female crossbred puppy was submitted to necropsy with a history of weakness and vomiting for several hours prior to death. The liver had multiple and slightly irregular yellowish foci up to 1.5 cm in diameter. Histologically, the liver parenchyma contained randomly distributed, occasionally coalescing hepatocellular necrosis, often accompanied by large numbers of mononuclear cells and neutrophils. Sections of liver stained by the Warthin-Starry silver impregnation technique revealed spiral- to curve-shaped bacteria predominantly located in bile canaliculi and occasionally in bile ducts. Aerobic culture of liver was negative, whereas small colonies were noted on Campylobacter selective media after 5 days of microaerobic incubation. The bacteria were gram negative and oxidase positive but catalase, urease, and indoxyl acetate negative; nitrate was not reduced to nitrite, and the organism did not hydrolyze hippurate. The bacteria were also resistant to 1.5% bile. Electron microscopy revealed spiral-shaped bacteria with bipolar sheathed flagella. By 16S rRNA analysis, the organism was determined to be H. canis. This is the first observation of H. canis in active hepatitis in a dog and correlates with recent findings of Helicobacter hepaticus- and Helicobacter bilis-related hepatic disease in mice. Further studies are clearly warranted to ascertain whether H. canis-associated hepatitis is more widespread in canines as well as a cause of previously classified idiopathic liver disease in humans.
基于生化、表型及16S rRNA分析，最近从正常和腹泻犬以及患肠胃炎的人类中分离出一种新型革兰氏阴性菌，被命名为犬幽门螺杆菌。一只2月龄雌性杂交幼犬因死前数小时出现虚弱和呕吐症状而被送检做尸检。肝脏有多个直径达1.5厘米、略不规则的淡黄色病灶。组织学检查显示，肝实质内有随机分布、偶尔融合的肝细胞坏死，常伴有大量单核细胞和中性粒细胞。用Warthin-Starry银浸染技术染色的肝脏切片显示，螺旋形至弯曲形细菌主要位于胆小管，偶尔见于胆管。肝脏需氧培养为阴性，而微需氧培养5天后在弯曲杆菌选择性培养基上发现了小菌落。该细菌革兰氏阴性、氧化酶阳性，但过氧化氢酶、脲酶和吲哚乙酸酯酶阴性；硝酸盐不还原为亚硝酸盐，且该菌不水解马尿酸盐。该细菌还对1.5%胆汁有抗性。电子显微镜显示有带双极鞘鞭毛的螺旋形细菌。通过16S rRNA分析，确定该菌为犬幽门螺杆菌。这是犬活动性肝炎中首次观察到犬幽门螺杆菌，与最近在小鼠中发现的与肝螺杆菌和胆螺杆菌相关的肝脏疾病相关。显然需要进一步研究以确定犬幽门螺杆菌相关性肝炎在犬类中是否更普遍，以及是否是人类先前分类的特发性肝病的病因。
