Panerai A E, Manfredi B, Granucci F, Sacerdote P
Department of Pharmacology, School of Medicine, University of Milan, Italy.
J Neuroimmunol. 1995 Apr;58(1):71-6. doi: 10.1016/0165-5728(94)00189-u.
In this study we show that the opioid peptide beta-endorphin exerts a tonic inhibitory effect on the proliferative response of splenocytes to the polyclonal mitogen phytohemoagglutinin throughout two separate sites of action: one central and one peripheral. The intracerebroventricular administration of beta-endorphin, in fact, induces a significant inhibition of splenocyte proliferation. In contrast, both the intracerebroventricular and the peripheral administration of anti-beta-endorphin gamma globulins induce a significant increase in proliferation. Moreover, an increase of splenocyte proliferation was observed also after the intravenous administration of gamma globulins and intraperitoneal naloxone, and this effect was still present in hypophysectomized rats. The data reported suggest that beta-endorphin exerts a tonic inhibitory effect on proliferation, acting centrally, and peripherally throughout a paracrine/autocrine mechanism. FACS experiments show that the effect observed is not the consequence of an alteration of lymphocyte trafficking induced by the opioid.
在本研究中，我们表明阿片肽β-内啡肽在两个不同的作用位点对脾细胞对多克隆有丝分裂原植物血凝素的增殖反应发挥着持续性抑制作用：一个是中枢位点，另一个是外周位点。事实上，脑室内注射β-内啡肽可显著抑制脾细胞增殖。相反，脑室内和外周注射抗β-内啡肽γ球蛋白均会导致增殖显著增加。此外，静脉注射γ球蛋白和腹腔注射纳洛酮后也观察到脾细胞增殖增加，且这种效应在垂体切除的大鼠中依然存在。所报道的数据表明，β-内啡肽通过中枢和外周的旁分泌/自分泌机制对增殖发挥持续性抑制作用。流式细胞术实验表明，观察到的效应并非阿片类药物诱导淋巴细胞转运改变的结果。
