Motegi K
Department of Medicine, Haramachi Red Cross Hospital, Gunma, Japan.
J Gastroenterol. 1995 Apr;30(2):224-30. doi: 10.1007/BF02348669.
Various anti-viral agents, e.g., interferon, have recently been used for the treatment of viral hepatitis. In the present study, duck hepatitis B virus (DHBV) was cultured in vitro and the anti-DHBV effects of acyclovir (ACV) and vidarabin 5'-monophosphate (VMP) were studied. The portal perfusion method was applied to the livers of 7-day-old white ducks weighing 100 g, bred in Japan, and hepatocytes were infected with DHBV in vitro. Duck hepatocytes infected with DHBV were cultured in medium containing ACV or VMP, and the anti-DHBV effects of these drugs were assessed by determining DHBV-DNA and duck hepatitis B surface antigen in the medium. Both ACV and VMP had anti-DHBV effects when used immediately after infection; however, both drugs were ineffective in hepatocytes obtained from a DHBV carrier duck. In conclusion, the anti-DHBV effects of these drugs were very limited. However, this culture system appears to be useful for studies of hepatitis virus and anti-viral drugs.
最近，多种抗病毒药物，如干扰素，已被用于治疗病毒性肝炎。在本研究中，鸭乙型肝炎病毒（DHBV）在体外进行培养，并研究了阿昔洛韦（ACV）和单磷酸阿糖腺苷（VMP）的抗DHBV作用。采用门静脉灌注法，对在日本饲养的7日龄、体重100克的白鸭肝脏进行处理，体外将肝细胞感染DHBV。将感染DHBV的鸭肝细胞在含有ACV或VMP的培养基中培养，并通过测定培养基中的DHBV-DNA和鸭乙型肝炎表面抗原评估这些药物的抗DHBV作用。ACV和VMP在感染后立即使用时均具有抗DHBV作用；然而，这两种药物对从DHBV携带鸭获得的肝细胞均无效。总之，这些药物的抗DHBV作用非常有限。然而，这种培养系统似乎对肝炎病毒和抗病毒药物的研究有用。
