Veenstra G J, Beumer T L, Peterson-Maduro J, Stegeman B I, Karg H A, van der Vliet P C, Destrée O H
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Mech Dev. 1995 Apr;50(2-3):103-17. doi: 10.1016/0925-4773(94)00328-k.
As a first step towards the elucidation of the role of the transcription factor Oct-1 in development, we prepared a monoclonal antibody to study the spatio-temporal distribution of Oct-1 protein in vivo. Here we report differential expression of the Oct-1 gene in the Xenopus embryo both at the RNA and the protein level. Transcripts and protein are detected in ectodermal and mesodermal cell lineages, in which the expression exhibits a pattern of progressive spatial restriction in the course of development. The Oct-1 expression as reported here is not correlated with cell density or cell proliferation in the embryo. Our results suggest a role of Oct-1 in the specification and differentiation of neuronal and neural crest cells. In many other cells, the developmental decision to down regulate Oct-1 is delayed, probably due to a high stability of the protein.
作为阐明转录因子Oct-1在发育过程中作用的第一步，我们制备了一种单克隆抗体，以研究Oct-1蛋白在体内的时空分布。在此我们报告Oct-1基因在非洲爪蟾胚胎的RNA和蛋白质水平上均有差异表达。转录本和蛋白质在外胚层和中胚层细胞谱系中被检测到，其中在发育过程中表达呈现出逐渐空间受限的模式。此处报道的Oct-1表达与胚胎中的细胞密度或细胞增殖无关。我们的结果表明Oct-1在神经元和神经嵴细胞的特化和分化中发挥作用。在许多其他细胞中，下调Oct-1的发育决定被延迟，这可能是由于该蛋白的高稳定性所致。
