de Pedro N, Céspedes M V, Delgado M J, Alonso-Bedate M
Departamento de Biología Animal II (Fisología Animal), Facultad de Ciencias Biológicas, Universidad Complutense, Madrid, Spain.
Regul Pept. 1995 May 4;57(1):77-84. doi: 10.1016/0167-0115(95)91255-4.
In the present study we examined the effects of intracerebroventricular (ICV) or intraperitoneal (IP) galanin administration on food intake in satiated goldfish, at different time intervals after injections, 0-2, 2-8 and 0-8 h. We found that food intake was increased by ICV administered galanin (1 and 3.33 micrograms) at 2 and 8 h postinjection, while no modifications on feeding were observed after intraperitoneal injection in any of the studied time intervals. The galanin receptor antagonist, galantide, blocked the galanin-induced feeding increase. Pretreatment with yohimbine (alpha 2-adrenergic receptor antagonist), but not with prazosin (alpha 1-adrenergic receptor antagonist) antagonized the stimulatory effect of galanin on ingestive behavior. These results suggest that galanin is involved in the central regulation of feeding in goldfish, being the food intake stimulatory effect mediated by the alpha 2-adrenergic system.
在本研究中，我们检测了脑室内（ICV）或腹腔内（IP）注射甘丙肽对饱足金鱼进食量的影响，注射后分别在0 - 2小时、2 - 8小时和0 - 8小时这几个不同时间间隔进行观察。我们发现，脑室内注射1微克和3.33微克甘丙肽后，在注射后2小时和8小时进食量增加，而在任何研究的时间间隔内，腹腔注射后均未观察到对进食有任何影响。甘丙肽受体拮抗剂丙谷酰胺可阻断甘丙肽诱导的进食增加。用育亨宾（α2 - 肾上腺素能受体拮抗剂）预处理可拮抗甘丙肽对摄食行为的刺激作用，而哌唑嗪（α1 - 肾上腺素能受体拮抗剂）预处理则无此作用。这些结果表明，甘丙肽参与金鱼进食的中枢调节，其促进进食的作用是由α2 - 肾上腺素能系统介导的。
