Molnár L, Baló-Banga J M, Horváth A, Leibinger J, Ablonczy E, Nováki M
Acta Physiol Acad Sci Hung. 1978;52(1):33-9.
A new two-step centrifugation technique is described for the rapid preparation of viable pure polymorphonuclear granulocytes from 9.5 ml of venous blood. The phagocytosis of these cells was stimulated by soluble DNA-anti-DNA complexes, produced in vitro by incubating high mol. wt. DNA with sera obtained from patients with severe SLE. The measure of phagocytosis was the amount of nitroblue-tetrazolium (NBT), reduced by the active microphages. The reduced NBT was estimated by counting the formazane positive cells or by extracting and measuring it spectrophotometrically. The immune-complex phagocytosis of control human granulocytes showed a linear relationship with the cell count per reaction mixture and displayed a bell-shaped dose-response curve as function of the immune-complex. The immune-complex phagocytosis by SLE granulocytes was decreased by about 40 per cent as compared to normal granulocytes.
本文描述了一种新的两步离心技术，可从9.5毫升静脉血中快速制备有活力的纯多形核粒细胞。这些细胞的吞噬作用由可溶性DNA-抗DNA复合物刺激，该复合物是通过将高分子量DNA与重症系统性红斑狼疮（SLE）患者的血清在体外孵育产生的。吞噬作用的衡量指标是活性巨噬细胞还原的硝基蓝四氮唑（NBT）的量。通过计数甲臜阳性细胞或通过分光光度法提取并测量来估计还原的NBT。对照人粒细胞的免疫复合物吞噬作用与每个反应混合物中的细胞计数呈线性关系，并作为免疫复合物的函数呈现钟形剂量反应曲线。与正常粒细胞相比，SLE粒细胞的免疫复合物吞噬作用降低了约40%。
