Sonza S, Maerz A, Uren S, Violo A, Hunter S, Boyle W, Crowe S
AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia.
AIDS Res Hum Retroviruses. 1995 Jul;11(7):769-76. doi: 10.1089/aid.1995.11.769.
Monocytes from HIV-seronegative persons were analyzed for CD4 expression and susceptibility to infection with HIV-1 on the day of isolation and following 1, 2, and 7 days in culture. Although surface CD4 was readily detected on freshly isolated monocytes, these cells were relatively resistant to infection. After 1 to 2 days in culture, when surface expression of CD4 had decreased over 90% to near background levels, cells became susceptible to infection with HIV-1. CD4 expression on monocytes cultured for 7 days was more than four times higher than that on freshly isolated cells, and the cultured cells were fully permissive to infection. These observations suggest that the differing susceptibility of monocytes and monocyte-derived macrophages to infection with HIV-1 is not simply proportional to the level of surface CD4 expression.
对来自HIV血清阴性者的单核细胞在分离当天以及培养1天、2天和7天后进行CD4表达分析和HIV-1感染易感性检测。尽管在新鲜分离的单核细胞上很容易检测到表面CD4，但这些细胞对感染相对有抗性。培养1至2天后，当CD4的表面表达下降超过90%至接近背景水平时，细胞变得易于感染HIV-1。培养7天的单核细胞上的CD4表达比新鲜分离细胞上的CD4表达高出四倍多，且培养细胞对感染完全敏感。这些观察结果表明，单核细胞和单核细胞衍生的巨噬细胞对HIV-1感染的不同易感性并非简单地与表面CD4表达水平成正比。
