Johansson M L, Sanni A, Lönner C, Molin G
Department of Food Technology, Lund University, Sweden.
Int J Food Microbiol. 1995 Apr;25(2):159-68. doi: 10.1016/0168-1605(94)00096-o.
One-hundred and twenty isolates of lactic acid bacteria isolated from ogi and three traditional cereal-based alcoholic beverages from Nigeria, together with 18 reference strains from Swedish sour doughs, and 50 type-and reference strains of mainly Lactobacillus, were phenotypically classified on their fermentation ability of 49 carbohydrates, including soluble starch. Data were examined by Jaccard Coefficient (SJ), Simple Matching Coefficient (SSM), and unweighted pair group algorithm with arithmetic averages (UPGMA). Seven major clusters were defined at the 82% SJ-similarity level (corresponds to the SSM-level of 91%). Three were identified as Lactobacillus plantarum or L. plantarum-like (together 41% of the ogi isolates). The others were obligately heterofermentative; Cluster 7 was identified as L. confusus (11% of the ogi isolates). Three minor clusters were identified as L. murinus, L. agilis or L. gallinarium, and Leuconostoc mesenteroides subsp. mesenteroides, respectively. The phenotype of the L. plantarum isolates varied within wide limits. Seventeen isolates possessed starch fermenting capacity. Nine of these were identified as L. plantarum or L. plantarum-like (isolated from ogi); one was identified as Leuconostoc mesenteroides subsp. mesenteroides, and the rest were unidentified non-clustering strains.
从尼日利亚的ogi和三种传统谷物基酒精饮料中分离出120株乳酸菌，连同来自瑞典酸面团的18株参考菌株，以及主要为乳酸杆菌属的50株模式菌株和参考菌株，根据它们对包括可溶性淀粉在内的49种碳水化合物的发酵能力进行表型分类。数据通过杰卡德系数（SJ）、简单匹配系数（SSM）和算术平均非加权配对组算法（UPGMA）进行分析。在82%的SJ相似性水平（对应于91%的SSM水平）定义了七个主要聚类。三个聚类被鉴定为植物乳杆菌或类植物乳杆菌（占ogi分离株的41%）。其他的是专性异型发酵菌；聚类7被鉴定为孔氏乳杆菌（占ogi分离株的11%）。三个次要聚类分别被鉴定为鼠乳杆菌、敏捷乳杆菌或鸡乳杆菌，以及肠膜明串珠菌肠膜亚种。植物乳杆菌分离株的表型在很宽的范围内变化。17株分离株具有淀粉发酵能力。其中9株被鉴定为植物乳杆菌或类植物乳杆菌（从ogi中分离）；1株被鉴定为肠膜明串珠菌肠膜亚种，其余为未鉴定的非聚类菌株。
