Lenander-Lumikari M, Johansson I, Vilja P, Samaranayake L P
Department of Cariology, University of Turku, Finland.
Oral Dis. 1995 Jun;1(2):86-91. doi: 10.1111/j.1601-0825.1995.tb00165.x.
Saliva is frequently used as a diagnostic fluid and several collection devices have been developed.
The aim of the present study was to investigate the validity and reliability of two types of Salivette collection kits (non-covered cotton roll and polypropylene covered polyether roll) relative to conventional collection of saliva using paraffin wax chewing stimulation.
Whole saliva samples were collected from 16 healthy volunteers. Following a cross-over design saliva was collected in a standardized way. The flow rate was determined and saliva samples were analyzed for pH, buffer capacity, electrolytes and protein/glycoprotein content.
We find that Salivette methods do not allow evaluation of flow rate. pH was unaffected but buffer capacity was lower in Salivette collected than in paraffin wax-stimulated saliva. The non-covered cotton rolls reduced the content of Na+, K+, Cl-, as well as glycoprotein markers (hexosamines, fucose, sialic acid), lysozyme, lactoferrin, salivary- and myeloperoxidase but increased the concentrations of Ca2+, PO4(3)- and SCN-. Polypropylene covered polyether rolls affected saliva composition less than the non-covered cotton rolls. Thus, SCN- and sIgA concentrations were higher and lysozyme activity lower in the former (covered roll) saliva than in paraffin wax saliva. The reliability of the Salivette kits was good.
We conclude that the Salivette method generates data significantly different from conventional paraffin wax-stimulated saliva such as buffer capacity and several electrolytes and organic components. Care should be taken in interpreting the results when such methods are employed.
唾液常被用作诊断液，并且已经开发了几种采集装置。
本研究的目的是调查两种类型的唾液采集管（无覆盖棉卷和聚丙烯覆盖聚醚卷）相对于使用石蜡咀嚼刺激常规采集唾液的有效性和可靠性。
从16名健康志愿者中采集全唾液样本。按照交叉设计以标准化方式采集唾液。测定流速，并分析唾液样本的pH值、缓冲能力、电解质和蛋白质/糖蛋白含量。
我们发现唾液采集管方法无法评估流速。pH值未受影响，但唾液采集管采集的唾液缓冲能力低于石蜡刺激的唾液。无覆盖棉卷降低了Na +、K +、Cl -以及糖蛋白标志物（己糖胺、岩藻糖、唾液酸）、溶菌酶、乳铁蛋白、唾液过氧化物酶和髓过氧化物酶的含量，但增加了Ca2 +、PO4(3)-和SCN -的浓度。聚丙烯覆盖聚醚卷对唾液成分的影响小于无覆盖棉卷。因此，前者（覆盖卷）唾液中的SCN -和分泌型免疫球蛋白A（sIgA）浓度较高，溶菌酶活性较低。唾液采集管试剂盒的可靠性良好。
我们得出结论，唾液采集管方法产生的数据与常规石蜡刺激的唾液有显著差异，如缓冲能力以及几种电解质和有机成分。使用此类方法时，在解释结果时应谨慎。
