Johansson I, Lenander-Lumikari M, Saellström A K
Department of Cariology, University of Umeå, Sweden.
J Dent Res. 1994 Jan;73(1):11-9. doi: 10.1177/00220345940730010101.
The composition of paraffin-stimulated and unstimulated whole saliva was compared between two groups of 8-12-year-old Indian children-one group with severe to moderate chronic protein-energy malnutrition (PEM group) and an age- and sex-matched control group with normal protein status or mild PEM. The classification of PEM was based on anthropometric measurements compared with Indian standards. Stimulated saliva was analyzed for the following variables: electrolytes (Na+, K+, Ca2+, Cl-, and PO4(3-)), total protein, hexosamines, fucose, sialic acid, and amylase. Unstimulated saliva samples were analyzed for total protein, salivary and myeloperoxidase, thiocyanate, lactoferrin, lysozyme, a bacteria-agglutinating protein (BAGP), total IgG, total IgA, and specific anti-S. mutans IgA. The results show that the PEM group had a reduced secretion rate of stimulated but not unstimulated saliva. Further, the Ca2+ and Cl- concentrations in stimulated saliva were significantly lower in the PEM group compared with the control group, but the other electrolyte levels were similar. No differences were found in total protein concentration or glycoprotein bound carbohydrates in stimulated saliva between the two groups, but the quantity of total protein secreted per min was reduced by 20% in the PEM group. Significantly lower levels of lactoferrin, BAGP, and anti-S mutans IgA were found in unstimulated saliva from children in the PEM group, but significantly higher levels of total IgG. We conclude that children with severe or moderate PEM, who have reduced secretion rate, buffer capacity, lower Ca2+, and protein secretion in stimulated saliva, also have impaired immunological and agglutinating defense factors in unstimulated saliva.
比较了两组8至12岁印度儿童石蜡刺激和未刺激的全唾液成分——一组为重度至中度慢性蛋白质-能量营养不良儿童（PEM组），另一组为年龄和性别匹配、蛋白质状态正常或轻度PEM的对照组。PEM的分类基于与印度标准相比的人体测量学指标。对刺激唾液分析了以下变量：电解质（Na+、K+、Ca2+、Cl-和PO4(3-)）、总蛋白、己糖胺、岩藻糖、唾液酸和淀粉酶。对未刺激唾液样本分析了总蛋白、唾液和髓过氧化物酶、硫氰酸盐、乳铁蛋白、溶菌酶、一种细菌凝集蛋白（BAGP）、总IgG、总IgA以及特异性抗变形链球菌IgA。结果显示，PEM组刺激唾液的分泌率降低，但未刺激唾液的分泌率未降低。此外，与对照组相比，PEM组刺激唾液中的Ca2+和Cl-浓度显著降低，但其他电解质水平相似。两组刺激唾液中的总蛋白浓度或糖蛋白结合碳水化合物无差异，但PEM组每分钟分泌的总蛋白量减少了20%。PEM组儿童未刺激唾液中的乳铁蛋白、BAGP和抗变形链球菌IgA水平显著降低，但总IgG水平显著升高。我们得出结论，重度或中度PEM儿童，其刺激唾液的分泌率、缓冲能力、Ca2+含量和蛋白质分泌降低，未刺激唾液中的免疫和凝集防御因子也受损。
