一种新型聚合酶链反应检测法显示唾液中幽门螺杆菌感染率高。
High prevalence of Helicobacter pylori in saliva demonstrated by a novel PCR assay.
作者信息
Li C, Musich P R, Ha T, Ferguson D A, Patel N R, Chi D S, Thomas E
机构信息
Department of Internal Medicine (Gastroenterology), James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37684, USA.
出版信息
J Clin Pathol. 1995 Jul;48(7):662-6. doi: 10.1136/jcp.48.7.662.
AIMS
To investigate the prevalence of Helicobacter pylori in the saliva of patients infected with this bacterium.
METHODS
A novel polymerase chain reaction (PCR) assay was developed to detect H pylori in saliva and gastric biopsy specimens from patients undergoing endoscopy.
RESULTS
Our PCR assay amplified a 417 base pair fragment of DNA from all 21 DNAs derived from H pylori clinical isolates but did not amplify DNA from 23 non-H pylori strains. Sixty three frozen gastric biopsy and 56 saliva specimens were tested. H pylori specific DNA was detected by PCR in all 39 culture positive biopsy specimens and was also identified from another seven biopsy specimens which were negative by culture but positive by histology. H pylori specific DNA was identified by PCR in saliva specimens from 30 (75%) of 40 patients with H pylori infection demonstrated by culture or histological examination, or both, and in three patients without H pylori infection in the stomach.
CONCLUSION
The results indicate that the oral cavity harbours H pylori and may be the source of infection and transmission.
目的
研究感染幽门螺杆菌的患者唾液中该菌的流行情况。
方法
开发了一种新型聚合酶链反应（PCR）检测法，用于检测接受内镜检查患者的唾液和胃活检标本中的幽门螺杆菌。
结果
我们的PCR检测法从所有21份源自幽门螺杆菌临床分离株的DNA中扩增出一段417碱基对的DNA片段，但未从23株非幽门螺杆菌菌株中扩增出DNA。对63份冷冻胃活检标本和56份唾液标本进行了检测。在所有39份培养阳性的活检标本中通过PCR检测到幽门螺杆菌特异性DNA，并且在另外7份培养阴性但组织学检查阳性的活检标本中也鉴定出该DNA。在40例经培养或组织学检查或两者均证实感染幽门螺杆菌的患者中，有30例（75%）的唾液标本通过PCR鉴定出幽门螺杆菌特异性DNA，在3例胃中未感染幽门螺杆菌的患者的唾液标本中也检测到该DNA。
结论
结果表明口腔中存在幽门螺杆菌，可能是感染和传播的来源。
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