Nakanishi T, Matsuo T, Ebisu S
Department of Conservative Dentistry, Tokushima University, School of Dentistry, Japan.
J Endod. 1995 Mar;21(3):131-6. doi: 10.1016/s0099-2399(06)80438-3.
The levels of immunoglobulins (IgG, IgA, and IgM) and inflammatory factors (elastase, prostaglandin E2, interleukin-1 alpha, interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha) in the blood of human dental pulp were quantified, using enzyme-linked immunosorbent assays. The pulpal blood samples were obtained with nylon fiber pellets from normal and inflamed dental pulp at pulp sites exposed on pulpectomy. Statistically significant differences between normal and inflamed pulp were found in the levels of IgG (p < 0.01), IgA (p < 0.05), IgM (p < 0.01), elastase (p < 0.05), and prostaglandin E2 (p < 0.01). These findings indicate that these factors play an important role in the pathogenesis of pulpal disease, and the sampling methods used in this study are useful for examination of pulpal inflammation.
采用酶联免疫吸附测定法对人牙髓血液中的免疫球蛋白（IgG、IgA和IgM）以及炎症因子（弹性蛋白酶、前列腺素E2、白细胞介素-1α、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α）水平进行定量。在牙髓摘除术中暴露的牙髓部位，用尼龙纤维小球从正常和发炎的牙髓获取牙髓血液样本。正常牙髓与发炎牙髓之间在IgG水平（p < 0.01）、IgA水平（p < 0.05）、IgM水平（p < 0.01）、弹性蛋白酶水平（p < 0.05）和前列腺素E2水平（p < 0.01）方面存在统计学上的显著差异。这些发现表明这些因素在牙髓疾病的发病机制中起重要作用，并且本研究中使用的采样方法对于牙髓炎症的检查是有用的。
