Wesierska-Gadek J, Hohenauer H, Hitchman E, Penner E
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
J Exp Med. 1995 Oct 1;182(4):1159-62. doi: 10.1084/jem.182.4.1159.
Patients with primary biliary cirrhosis frequently develop autoantibodies directed to gp210, a major glycoprotein of the nuclear pore complex. This protein contains a large glycosylated cisternal domain, a single transmembrane segment, and a short cytoplasmic tail. It has been previously shown that autoantibodies from primary biliary cirrhosis patients exclusively react with the cytoplasmic tail. We demonstrate that autoantibodies against gp210 recognize at least two different epitopes. 4 out of 12 anti-gp210 positive sera reacted with the fragment consisting of the cytoplasmic tail, and 8 sera targeted a novel epitope located within the large glycosylated lumenal domain. Moreover, our data prove that carbohydrate moieties are an essential part of this novel epitope. We propose, therefore, that future screening assays should be performed with antigens possessing both epitopes to detect all sera with anti-gp210 specificity.
原发性胆汁性肝硬化患者经常会产生针对核孔复合体主要糖蛋白gp210的自身抗体。该蛋白包含一个大的糖基化池结构域、一个单一的跨膜片段和一个短的胞质尾。先前已经表明，来自原发性胆汁性肝硬化患者的自身抗体仅与胞质尾发生反应。我们证明，针对gp210的自身抗体识别至少两个不同的表位。12份抗gp210阳性血清中有4份与由胞质尾组成的片段发生反应，8份血清靶向位于大的糖基化管腔结构域内的一个新表位。此外，我们的数据证明碳水化合物部分是这个新表位的重要组成部分。因此，我们建议，未来的筛查试验应该用具有这两个表位的抗原进行，以检测所有具有抗gp210特异性的血清。
