Umehara K
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Nihon Shokakibyo Gakkai Zasshi. 1995 Aug;92(8):1161-8.
To clarify the role of nitric oxide (NO) in the pancreas, we investigate the localization of NADPH-diaphorase activity and neuronal nitric oxide synthase (nNOS) immunoreactivity in the pancreas of the rat and dog. NADPH-diaphorase activity and nNOS immunoreactivity were identical in the neuronal element of both species. NADPH-diaphorase activity and nNOS immunoreactivity were localized in neurons and endothelial cells of vascular system. Numerous NADPH-diaphorase positive and nNOS immunoreactive fibers were observed around pancreatic ducts and arteries. A moderate number of NADPH-diaphorase positive and nNOS immunoreactive fibers were observed surrounding the acini of dog pancreas, but there were few of these structures in rat pancreas. The islets of the rat pancreas contained a moderate number of NADPH-diaphorase positive and nNOS immunoreactive fibers. However in the dog, these positive fibers were not detected inside the islets. In the rat pancreas, 85% of the ganglion cells showed NADPH-diaphorase staining. In the dog, however, 30-50% of the ganglion cells demonstrated NADPH-diaphorase activity. Although NADPH-diaphorase histochemistry did not show any positive staining in the islet cells of pancreas in either species, NOS immunocytochemical method demonstrated weak positive staining in the rat islet cells. These results indicate that NO may play an important role for the neuronal regulation of pancreatic exocrine and endocrine activities in both species, but in the species-specific manner.
为阐明一氧化氮（NO）在胰腺中的作用，我们研究了大鼠和犬胰腺中还原型辅酶Ⅱ黄递酶（NADPH-d）活性及神经元型一氧化氮合酶（nNOS）免疫反应性的定位。两种动物的神经成分中，NADPH-d活性和nNOS免疫反应性是相同的。NADPH-d活性和nNOS免疫反应性定位于血管系统的神经元和内皮细胞。在胰腺导管和动脉周围观察到大量NADPH-d阳性和nNOS免疫反应性纤维。在犬胰腺腺泡周围观察到中等数量的NADPH-d阳性和nNOS免疫反应性纤维，但在大鼠胰腺中这类结构较少。大鼠胰腺胰岛含有中等数量的NADPH-d阳性和nNOS免疫反应性纤维。然而在犬中，未在胰岛内部检测到这些阳性纤维。在大鼠胰腺中，85%的神经节细胞显示NADPH-d染色。然而在犬中，30%-50%的神经节细胞表现出NADPH-d活性。尽管NADPH-d组织化学在两种动物的胰腺胰岛细胞中均未显示任何阳性染色，但NOS免疫细胞化学方法在大鼠胰岛细胞中显示弱阳性染色。这些结果表明，NO可能在两种动物的胰腺外分泌和内分泌活动的神经调节中发挥重要作用，但方式具有种属特异性。
