酵母聚合酶I转录终止子的释放元件可独立于Reb1p发挥作用。
The release element of the yeast polymerase I transcription terminator can function independently of Reb1p.
作者信息
Jeong S W, Lang W H, Reeder R H
机构信息
Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
出版信息
Mol Cell Biol. 1995 Nov;15(11):5929-36. doi: 10.1128/MCB.15.11.5929.
Abstract
The Saccharomyces cerevisiae polymerase I (polI) transcription terminator utilizes a DNA-binding protein (Reb1p) as part of a signal that causes the polymerase to pause prior to release from the template. To study the release element of the terminator, independent of the Reb1p pause signal, we have replaced the Reb1p binding site with the binding site for the lac repressor, which acts as a self-contained heterologous pause signal for polI. Release efficiency is maximal when the lac repressor causes polI to pause in exactly the same position that Reb1p would have caused it to pause, suggesting that polI must be precisely positioned for transcript release to occur. Mutational analysis shows that the release element is a region rich in T residues which codes for the extreme 3' end of the transcript and which has no apparent ability to form hairpins when transcribed into RNA. We discuss possible mechanisms whereby this polI release element might function.
摘要
酿酒酵母聚合酶I（polI）转录终止子利用一种DNA结合蛋白（Reb1p）作为信号的一部分，该信号导致聚合酶在从模板释放之前暂停。为了研究终止子的释放元件，独立于Reb1p暂停信号，我们用乳糖阻遏物的结合位点取代了Reb1p结合位点，乳糖阻遏物作为polI的一个独立的异源暂停信号。当乳糖阻遏物使polI在与Reb1p使它暂停的完全相同位置暂停时，释放效率最高，这表明polI必须精确定位才能发生转录本释放。突变分析表明，释放元件是一个富含T残基的区域，它编码转录本的极端3'端，当转录成RNA时没有明显形成发夹的能力。我们讨论了这种polI释放元件可能发挥作用的机制。
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