Ribas G, Frenzilli G, Barale R, Marcos R
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Mutat Res. 1995 Aug;344(1-2):41-54. doi: 10.1016/0165-1218(95)90037-3.
The genotoxicity of the herbicides, alachlor, atrazine, maleic hydrazide, paraquat and trifluralin has been evaluated in the single-cell gel electrophoresis (SCGE) assay by using human peripheral blood lymphocytes. All treatments were conducted with and without the presence of an external bioactivation source (S9 mix). The results indicate that all the herbicides tested are able to give positive results by increasing the comet tail length, which would confirm both the genotoxicity of the herbicides and the sensitivity of the assay in front of these chemicals. Alachlor and atrazine give similar results in treatments with and without S9, while when the S9 mix was not used paraquat and trifluralin genotoxicity was higher. On the other hand, although maleic hydrazide genotoxicity was higher when S9 mix was used at normal pH (7.4), our data show that its genotoxicity depends largely on the pH solution, increasing as the pH decreased.
已通过使用人外周血淋巴细胞的单细胞凝胶电泳（SCGE）试验评估了除草剂甲草胺、莠去津、马来酰肼、百草枯和氟乐灵的遗传毒性。所有处理均在有和没有外部生物活化源（S9混合物）的情况下进行。结果表明，所有测试的除草剂都能够通过增加彗星尾长给出阳性结果，这将证实除草剂的遗传毒性以及该试验对这些化学物质的敏感性。在有和没有S9的处理中，甲草胺和莠去津给出相似的结果，而当不使用S9混合物时，百草枯和氟乐灵的遗传毒性更高。另一方面，尽管在正常pH（7.4）下使用S9混合物时马来酰肼的遗传毒性更高，但我们的数据表明其遗传毒性在很大程度上取决于pH溶液，随着pH降低而增加。
