腐殖酸对培养的人淋巴细胞的遗传毒性及其与除草剂甲草胺和马来酰肼的相互作用。

Genotoxicity of humic acid in cultured human lymphocytes and its interaction with the herbicides alachlor and maleic hydrazide.

作者信息

Ribas G, Carbonell E, Creus A, Xamena N, Marcos R

机构信息

Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, Spain.

出版信息

Environ Mol Mutagen. 1997;29(3):272-6. doi: 10.1002/(sici)1098-2280(1997)29:3<272::aid-em7>3.0.co;2-e.

DOI:10.1002/(sici)1098-2280(1997)29:3<272::aid-em7>3.0.co;2-e

PMID:9142170

Abstract

The genotoxicity of humic acid and its possible interaction with the herbicides alachlor and maleic hydrazide have been evaluated in cultured human lymphocytes from two donors. Humic acid and the two herbicides have been tested (alone and combined) for sister-chromatid exchange (SCE) induction. In addition, the effect of two different preincubation times, 2 and 24 hr, was analyzed. The results indicate that humic acid and the herbicides alachlor and maleic hydrazide appear to significantly enhance the frequency of SCE, the effect of the herbicides being more pronounced. With reference to the possible interaction of humic acid with the herbicides, the results do not show a common pattern, although mainly an additive effect was obtained. Nevertheless, there is some evidence suggesting that antagonism may occur, especially in the combined treatment of humic acid and maleic hydrazide.

摘要

已在来自两名供体的培养人淋巴细胞中评估了腐殖酸的遗传毒性及其与除草剂甲草胺和马来酰肼的可能相互作用。已测试了腐殖酸和两种除草剂（单独及联合使用）对姐妹染色单体交换（SCE）的诱导作用。此外，还分析了两种不同预孵育时间（2小时和24小时）的影响。结果表明，腐殖酸以及除草剂甲草胺和马来酰肼似乎能显著提高SCE频率，除草剂的作用更为明显。关于腐殖酸与除草剂的可能相互作用，结果未显示出共同模式，尽管主要获得的是相加效应。然而，有一些证据表明可能会发生拮抗作用，尤其是在腐殖酸和马来酰肼联合处理时。