Musk S R, Clapham P, Johnson I T
Institute of Food Research, Norwich Laboratory, Norwich Research Park, UK.
Food Chem Toxicol. 1997 Mar-Apr;35(3-4):379-85. doi: 10.1016/s0278-6915(97)00120-8.
Two compounds found in garlic, diallyl sulfide (DAS) and diallyl disulfide (DDS), were tested for cytotoxic and genotoxic effects in a Chinese hamster ovary cell line. DDS was found to be more cytotoxic than DAS (showing a Dq of 1.6 micrograms/ml and a D0 of 0.6 microgram/ml as opposed to values of 295 and 90 micrograms/ml, respectively). Both compounds were found to induce both chromosome aberrations and sister chromatid exchanges (SCEs) with DDS again being more active on a weight-for-weight basis, exhibiting activity at concentrations below 10 micrograms/ml compared with the levels of 300 micrograms/ml and above required for DAS to show any effect. The addition of rat liver S-9 activation fraction to the assays modified the effects of the two compounds in a non-consistent manner. It reduced the induction of SCEs by both compounds, enhanced the generation of aberrations by DDS (but not by DAS) and radically altered the parameters of both survival curves, reducing the Dq values almost to zero but increasing the D0 values.
对大蒜中发现的两种化合物二烯丙基硫醚（DAS）和二烯丙基二硫醚（DDS）在中国仓鼠卵巢细胞系中进行了细胞毒性和遗传毒性作用测试。发现DDS比DAS具有更强的细胞毒性（Dq值为1.6微克/毫升，D0值为0.6微克/毫升，而DAS的相应值分别为295和90微克/毫升）。发现这两种化合物均能诱导染色体畸变和姐妹染色单体交换（SCE），同样基于重量计算，DDS的活性更强，与DAS显示任何效应所需的300微克/毫升及以上的浓度相比，DDS在低于10微克/毫升的浓度下就表现出活性。在试验中添加大鼠肝脏S-9活化组分对这两种化合物的效应产生了不一致的改变。它降低了两种化合物对SCE的诱导，增强了DDS（而非DAS）对畸变的产生，并且从根本上改变了两条存活曲线的参数，将Dq值几乎降低至零，但提高了D0值。
