Gemmill R M, Chumakov I, Scott P, Waggoner B, Rigault P, Cypser J, Chen Q, Weissenbach J, Gardiner K, Wang H
Eleanor Roosevelt Institute, Denver, Colorado 80206, USA.
Nature. 1995 Sep 28;377(6547 Suppl):299-319. doi: 10.1038/377299a0.
A map of human chromosome 3 which integrates both physical and genetic data has been developed from the fusion of two large collections of markers and corresponding yeast artificial chromosome (YAC) clones. The map contains 972 megabase-sized YACs identified with 593 primary markers, of which 162 are highly polymorphic sequence-tagged sites (STSs) and form a closely spaced genetic linkage map; the remaining markers are hybridization-based. Chromosome 3 is now represented by 24 large YAC contigs whose order and orientation is largely known. The map generated by fusion of these hybridization- and STS-based datasets covers about 80% (over 160 megabases) of the chromosome and will provide the foundation necessary for rapid development of a detailed genetic understanding for this large autosome.
通过融合两个大型标记集合及相应的酵母人工染色体（YAC）克隆，绘制出了整合物理和遗传数据的人类3号染色体图谱。该图谱包含972个以593个主要标记鉴定的兆碱基大小的YAC，其中162个是高度多态性的序列标签位点（STS），形成了一个紧密排列的遗传连锁图谱；其余标记基于杂交。3号染色体现在由24个大型YAC重叠群表示，其顺序和方向大多已知。通过融合这些基于杂交和STS的数据集生成的图谱覆盖了该染色体约80%（超过160兆碱基）的区域，并将为快速深入了解这条大型常染色体提供必要的基础。
