Andersson K, Hansson U B, Björklund J, Alkner U
Department of Biochemistry, Lund University, Sweden.
Scand J Immunol. 1995 Oct;42(4):407-17. doi: 10.1111/j.1365-3083.1995.tb03674.x.
In this investigation, the modulating effects of non-immune human IgG and rheumatoid factors (RFs) on antigen-antibody complexations were studied. Non-immune human IgG, as well as RF, were found to inhibit the binding of antigen to specific antibodies of both human and rabbit origin. In addition, human immunoglobulins were also able to modify the composition of preformed antigen-antibody complexes. The effects were detected by immunological methods in two different antigen-antibody systems (human serum albumin-rabbit anti-HSA and tetanus toxoid-human anti-TT). Changes in biological activities could be followed by employing enzymes (glucose-6-phosphate dehydrogenase and human placental alkaline phosphatase) as antigens. The outcome of the effects was found to be dependent on the ratio of antigen to antibody, the antigen-binding properties of the antibody and its origin, and on the properties of the immunoglobulins added. The observed changes could not be explained only by the presence of specific antibodies in the immunoglobulin preparations. The ability of immunoglobulins to modulate antigen-antibody complexations may provide a rationale for the large amounts of non-specific immunoglobulins in the circulation by preventing premature precipitation and promoting the elimination of antigenic molecules.
在本研究中，研究了非免疫性人IgG和类风湿因子（RFs）对抗原-抗体复合物形成的调节作用。发现非免疫性人IgG以及RF均能抑制抗原与人和兔来源的特异性抗体的结合。此外，人免疫球蛋白还能够改变预先形成的抗原-抗体复合物的组成。通过免疫方法在两种不同的抗原-抗体系统（人血清白蛋白-兔抗HSA和破伤风类毒素-人抗TT）中检测到了这些作用。采用酶（葡萄糖-6-磷酸脱氢酶和人胎盘碱性磷酸酶）作为抗原，可以追踪生物活性的变化。发现这些作用的结果取决于抗原与抗体的比例、抗体的抗原结合特性及其来源，以及所添加免疫球蛋白的特性。观察到的变化不能仅通过免疫球蛋白制剂中存在特异性抗体来解释。免疫球蛋白调节抗原-抗体复合物形成的能力可能为循环中大量非特异性免疫球蛋白提供了一个理论依据，即通过防止过早沉淀和促进抗原分子的清除。
