Guobadia E E, Fagbemi B O
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Vet Parasitol. 1995 Jun;58(3):247-53. doi: 10.1016/0304-4017(94)00721-n.
The time-course analysis of the antibody response to Fascicola gigantica infection in sheep was studied by enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunoelectrotransfer blot (EITB). Sera from sheep experimentally infected with F. gigantica were reacted with excretory-secretory antigens of the worm before and after chemotherapy with oxyclozanide. In ELISA, there was a significant increase in anti-Fasciola antibody by 2 weeks after infection and there was a sharp decrease in antibody titer by 4 weeks after treatment. By EITB, the infected sheep sera recognised four polypeptides in the range of 43-75 kDa as early as 2 weeks after infection, with more polypeptides being recognised as the infection progressed. Recognition of an 87 kDa antigen was lost by 2 weeks after treatment and is therefore a good marker for treatment efficacy. Comparative immunoblotting with sheep anti-Paramphistomum, anti-Dicrocoelium and anti-Fasciola sera revealed that the 17 kDa, 21 kDa, 57 kDa and 69 kDa proteins are specific to fasciolosis and are good antigens for early and specific immunodiagnosis of F. gigantica infection in sheep.
采用酶联免疫吸附测定（ELISA）和酶联免疫电转移印迹法（EITB）研究了绵羊对巨片形吸虫感染的抗体反应的时间进程分析。用氧氯苯酰对感染巨片形吸虫的绵羊进行化疗前后，将其血清与该蠕虫的排泄-分泌抗原反应。在ELISA中，感染后2周抗片形吸虫抗体显著增加，治疗后4周抗体滴度急剧下降。通过EITB，感染绵羊血清早在感染后2周就识别出43-75 kDa范围内的四种多肽，随着感染进展，识别出更多多肽。治疗后2周失去了对87 kDa抗原的识别，因此它是治疗效果的良好标志物。用绵羊抗双口吸虫、抗片形吸虫和抗肝片吸虫血清进行的比较免疫印迹显示，17 kDa、21 kDa、57 kDa和69 kDa蛋白质是片形吸虫病特有的，是绵羊巨片形吸虫感染早期特异性免疫诊断的良好抗原。
